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Impact of pituitary FSH purification on in vitro early folliculogenesis in goats Biocell
Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R..
Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003
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Limb immobilization alters functional electrophysiological parameters of sciatic nerve BJMBR
Alves,J.S.M.; Leal-Cardoso,J.H.; Santos-Júnior,F.F.U.; Carlos,P.S.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Ceccatto,V.M.; Barbosa,R..
Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Sciatic nerve; Compound action potential; Conduction velocity; Rheobase; Chronaxy.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715
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